| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Conley, Sarah L (14)
|
NITWST |
29.34 |
28.70 |
-0.64 |
| 2 |
Bubar, Carolyn M (14)
|
NITWST |
34.53 |
30.44 |
-4.09 |
| 3 |
Wood, Mya E (13)
|
NITWST |
31.10 |
30.73 |
-0.37 |
| 4 |
Choroser, Beatrice C (14)
|
NITWST |
31.54 |
31.10 |
-0.44 |
| 5 |
Clouser, KaRynn F (14)
|
NIBCSR |
35.23 |
31.41 |
-3.82 |
| 6 |
Ormond, Kaleigh J (14)
|
NIUN-H |
33.97 |
33.66 |
-0.31 |
| 7 |
Musterait, Madeline A (13)
|
NITWST |
33.70 |
34.16 |
0.46 |
| 8 |
Hodge, Emily E (14)
|
NIHAMB |
45.48 |
39.10 |
-6.38 |
| 9 |
Astalos, Savannah T (14)
|
NIHAMB |
47.27 |
42.22 |
-5.05 |
| 10 |
Pattison, Kennedy E (13)
|
NIHHSC |
|
51.92 |
- |